Similarly, pharmacological doses of ascorbate in C57Bl/6NHsd mice prevented radiation-induced (15Gy/1 fx) alopecia and achromotrichia [221]. The H2O2 produced is also able to rapidly interact with catalytically active ferrous iron in classical Fenton chemistry (Equation (4)) to produce the highly reactive hydroxyl radical (HO) and cause further cellular damage. 3). For b-h, n=3 biological replicates; error bars are S.D. WebSodium selenite (SSe) is the most widely-distributed inorganic selenium in nature. Selenite by virtue of oxidizing cell membrane thiols, can prevent the formation of the coat and consequently makes cancer cells vulnerable to the immune surveillance and destruction. This study investigated the mechanism of action of sodium selenite in human prostate cancer PC-3 cells using proteomics. Chen W.Y., Giobbie-Hurder A., Gantman K., Savoie J., Scheib R., Parker L.M. d, Quantification of CC3 positive cells over total cells in CTRL and SEPHS2 deficient MDAMB231 and U251. Since then, more studies have come out suggesting that the RDA should be increased to 75 mcg/d. Methods 11, 783784 (2014). After a median time of 7.5 years, the subjects taking vitamin C supplements had lower total cancer incidence compared to controls [185]. The mechanism of selenite penetrates in the cell is shown in Figure 3 in this paper. We thank M. Green, E. Baehrecke, A. Mercurio, D. Sabatini, C. Haynes, D. Guertin, B. Bible, K. Krupczak, K. Luk, C. Brown, A. Jaferi and S. Deibler for advice, assistance and feedback. Scale bar=20m. Lim Y., Traber M.G. Allen B.G., Bodeker K.L., Smith M.C., Monga V., Sandhu S., Hohl R. First-in-Human phase I clinical trial of pharmacologic ascorbate combined with radiation and temozolomide for newly diagnosed glioblastoma. sharing sensitive information, make sure youre on a federal Scale bar=100m. However, the pharmacological mechanism of the anticancer action of sodium selenite in peritoneal cancer cells remains unclear. Proc. Selenite, a representative inorganic form of selenium, is preferentially accumulated in tumors. Drug-tolerant persister cancer cells are vulnerable to GPX4 inhibition. 8 Reactive oxygen species production by excess selenium, and implications in SEPHS2 KO toxicity. Unauthorized use of these marks is strictly prohibited. The cBio cancer genomics portal: an open platform for exploring multidimensional cancer genomics data. Melatonin has been shown to mitigate the activity of the heme containing protein, myeloperoxidase [27]. Metabolism of selenite in human lung cancer cells: X-ray absorption and fluorescence studies. Dong H, Zeng L, Wu Y, Miao S, Ni N, Liu N, Chen W, DU J. Nan Fang Yi Ke Da Xue Xue Bao. For f and g, if no tumor has formed, tumor weight and volume was designated 0 for CTRL, n=6 and for SEPHS2 KO n=2. h, Immunoblot of SEPHS2 in CTRL and SEPHS2 KO MDAMB231 orthotopic xenograft tumors. eCollection 2022. All raw data that support the findings of this study are available from the corresponding author upon request. The experiment was carried out in two different modes to modulate intracellular reduced glutathione (GSH) content. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. 2017 Oct 24;6(4):39. doi: 10.3390/cells6040039. and D.K. Epub 2022 Aug 5. The supplementation of melatonin to adriamycin-therapy returned hepatic and cardiac function markers, circulating iron markers, and TBARS and protein carbonyl levels to baseline levels. Sign up for the Nature Briefing: Cancer newsletter what matters in cancer research, free to your inbox weekly. Rhee S., Kang S., Chang T., Jeong W., Kim K. Peroxiredoxin, a novel family of peroxidases. e, Total thiol quantification of 24hr conditioned media from control and SLC3A2 KO U251 cells. WebSodium selenite has been utilized in studies of cell proliferation and cancer research.4,5 It has been used to alter gene expression in HepG2 cells as analyzed by cDNA microarrays.6 Sodium selenite can inhibit zinc finger protein/DNA interactions.7 Sodium selenite has been shown to alter mitochondrial membrane This site needs JavaScript to work properly. For a,c,e, n=3 biological replicates; error bars are S.D. J Am Chem Soc. The anticancer mechanisms of ascorbate have been well elucidated in comparison to other redox active small molecules. The biology of oxygen radicals. SEPHS2 is required in cancer cells to detoxify selenide, an intermediate that is formed during selenocysteine biosynthesis. Tocharus J., Khonthun C., Chongthammakun S., Govitrapong P. Melatonin attenuates methamphetamine-induced overexpression of pro-inflammatory cytokines in microglial cell lines. WebSodium selenite has been utilized in studies of cell proliferation and cancer research.3,4 It has been used to alter gene expression in HepG2 cells as analyzed by cDNA microarrays. The left lower quadrant represents Annexin V negative and PI negative live cells. Patients were treated with high doses of SLM, as high as 4mg, twice daily for 2 weeks then once a day in combination with axitinib, without a dose limiting toxicity (DLT), and yet with promising efficacy signal [176]. Aykin-Burns N., Pathak R., Boerma M., Kim T., Hauer-Jensen M. Utilization of vitamin E analogs to protect normal tissues while enhancing antitumor effects. Clipboard, Search History, and several other advanced features are temporarily unavailable. Glucose Limitation Sensitizes Cancer Cells to Selenite-Induced Cytotoxicity via SLC7A11-Mediated Redox Collapse. WebSodium selenite (SS) affects cancer cells cultured as multicellular tumor spheroids (MCTS or 3D). In this review we summarize the various activities of Se compounds and focus on their relation to DNA damage and repair. eCollection 2022. Amoedo, N. D., Obre, E. & Rossignol, R. Drug discovery strategies in the field of tumor energy metabolism: limitations by metabolic flexibility and metabolic resistance to chemotherapy. J. Biol. Schoenfeld J.D., Sibenaller Z.A., Mapuskar K.A., Bradley M.D., Wagner B.A., Buettner G.R. AscH acts as a reducing agent and donor antioxidant that can undergo two consecutive one-electron oxidations to produce the ascorbate radical (Asc-) and dehydroascorbic acid (DHA), respectively. e, Immunoblots of CAT and SEPHS2 in U251 and MDAMB231 cells overexpressing blank vector or CAT and subjected to KO with guides against SEPHS2 for 9 days. This secondary analysis aimed to explore the metabolic effects of selenium on patients with BCRL. Ascorbate with radiation and temozolomide, Progression-free survival 13.3 months; average overall survival 21.5 months. Neurotoxins: free radical mechanisms and melatonin protection. Vijayalaxmi Reiter R., Herman T., Meltz M. Melatonin reduces gamma radiation-induced primary DNA damage in human blood lymphocytes. WebSodium selenite is a precursor of Sec biosynthesis, and supplement of cells with Na 2 SeO 3 could significantly increases the Sec level in cells (Kryukov et al., 2003). The epigenetically regulated Osseni R.A., Rat P., Bogdan A., Warnet J.-M., Touitou Y. Se-methylselenocysteine sensitizes hypoxic tumor cells to irinotecan by targeting hypoxia-inducible factor 1alpha. Alim, I. et al. Bonnefont-Rousselot D., Collin F., Jore D., Gards-Albert M. Reaction mechanism of melatonin oxidation by reactive oxygen species in vitro. PubMedGoogle Scholar. These molecules have been shown to possess anticancer activity in several cancer cell lines, including prostate, breast, lung, hepatoma, bladder, and osteosarcoma [ 52 ]. The profiles of DNA methylation, microRNA (miRNA), long non-coding RNA (lncRNA), and message RNA (mRNA) in breast cancer cells treated with sodium selenite were examined by microarrays. To correct this problem a number of organic and inorganic selenium compounds were developed and tested. 8600 Rockville Pike aDepartment of Radiation Oncology, University of Iowa, Iowa City, IA, USA, bDepartment of Cancer Biology, University of Iowa, Iowa City, IA, USA, cDivision of Hematology and Oncology, Department of Internal Medicine, University of Iowa, Iowa City, IA, USA. Qian S., Wang H., Schafer F., Buettner G. EPR detection of lipid-derived free radicals from PUFA, LDL, and cell oxidations. Levine M., Conry-Cantilena C., Wang Y., Welch R., Washko P., Dhariwal K. Vitamin C pharmacokinetics in healthy volunteers: evidence for a recommended dietary allowance. Schoenfeld J.D., Sibenaller Z.A., Mapuskar K.A., Wagner B.A., Cramer-Morales K.L., Furqan M. O(2)(-) and H(2)O(2)-mediated disruption of Fe metabolism causes the differential susceptibility of NSCLC and GBM cancer cells to pharmacological ascorbate. Epub 2021 Mar 15. Soc. Subcutaneous -TE (75100mgkg1) given to mice before 12Gy TBI protected the animals from TBI-induced gastrointestinal damage, as evidenced by an increased number of jejunal crypt cells present 30 days post-radiation relative to radiation alone [154]. Ann Hematol. This site needs JavaScript to work properly. Activation of this antioxidant network has been shown to reduce intracellular ROS levels and promote cellular differentiation in prostate cancer cells [5,10,39,40]. For all panels, the measure of center is mean. Fisher A.B. A.E.C., N.L. Epub 2021 Sep 6. HHS Vulnerability Disclosure, Help 17, 4564 (1994). This work was supported by the Suh Kyungbae Foundation (SUHF) Young Investigator Award to D.K. Muecke R., Micke O., Schomburg L., Glatzel M., Reichl B., Kisters K. Multicenter, phase III trial comparing selenium supplementation with observation in gynecologic radiation oncology: follow-up analysis of the survival data 6 Years after cessation of randomization. Bonetti G, Dhuli K, Michelini S, Michelini S, Michelini S, Ricci M, Cestari M, Bertelli M. J Prev Med Hyg. Go to patients with clinically documented lymphedema of upper limb secondary to breast cancer management (surgery - axillary nodal dissection, and radiotherapy) patients who have had other modalities of management can be included, e.g. The solvent and treatment regimen of sodium selenite cause its effects to vary on the radiation response of human bronchial cells from tumour and normal tissues. However, selenium treatment may affect WebEpidemiological and clinical data suggest that selenium may prevent prostate cancer, but the biological effects of selenium on normal or malignant prostate cells are not well known. Stevens R.G. The https:// ensures that you are connecting to the [105]. Weekley CM, Aitken JB, Vogt S, Finney LA, Paterson DJ, de Jonge MD, Howard DL, Witting PK, Musgrave IF, Harris HH. From each of these studies, the authors concluded that melatonin reduced the risk of death at 1 year (relative risk=0.66, 95% confidence interval: 0.590.73) with no severe adverse events. A.N.M.-O. A phase I safety, pharmacokinetic, and pharmacodynamic presurgical trial of vitamin E -tocotrienol in patients with pancreatic ductal neoplasia. Treatment with sodium selenite lowers reactive oxygen species (ROS) production, causes a spontaneous reduction in lymphedema volume, increases the efficacy of physical therapy for lymphedema, and reduces the incidence of erysipelas infections in patients with chronic lymphedema. As a result, apoptosis can be readily observed in cells treated with single digit M concentrations of sodium selenite. Extended Data Fig. To determine whether sodium selenite during radiotherapy protects not only normal cells but also cancer cells, which would imply a reduction of the desired effect of irradiation on tumour during WebA significant percentage of cancer patients develop secondary lymphedema after surgery or radiother-apy. Stevens R.G., Brainard G.C., Blask D.E., Lockley S.W., Motta M.E. Yang JC, Huang LH, Wu SC, Kuo PJ, Wu YC, Wu CJ, Lin CW, Tsai PY, Hsieh CH. and D.K. However, its mechanism of action remains unclear. The unique character of selenium compounds, including sodium selenite and Se-methylselenocysteine (MSC), is that they exert cytotoxic effects on neoplastic cells, providing a great potential for treating cancer cells being highly resistant to cytostatic drugs. f, Weight measurements for ex vivo CTRL and SEPHS2 KO MDAMB231 orthotopic xenograft tumors. The right hand panels are magnifications of the regions outlined by the white square. Biophys. Mol. K.S. and transmitted securely. b, Validation of specificity of GPX4 antibody in CTRL and SEPHS2 KO MDAMB231 cell pellet block by immunohistochemistry. Am. Tocotrienols have also been shown to accumulate selectively in cancer cells relative to normal cells following oral administration [96,97]. Results As recently demonstrated, selenite is not an antioxidant, but possesses oxidizing properties in the presence of specific substrates. Immunohistochem. A different isoform in the GPx family, GPx1, also has Se at its active site. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Superoxide dismutase in redox biology: the roles of superoxide and hydrogen peroxide. Candidate diagnostic markers and tumor suppressor genes for adrenocortical carcinoma by expression profile of genes on chromosome 11q13. wrote the manuscript with consultation from all of the authors. Novel HDAC11 inhibitors suppress lung adenocarcinoma stem cell self-renewal and overcome drug resistance by suppressing Sox2. While GPx4 appears to play a major role in the Se-dependent antioxidant effect of lipid peroxidation, other Se-dependent enzymes should not be overlooked. In this study, we investigated the anti-tumor activity of sodium selenite (Na 2 SeO 3) and explored its possible mechanisms involved in a breast cancer cell line.We found that Na 2 SeO 3 could inhibit the cell viability of MCF7 cells, yet with minimal damage to human umbilical vein Glutathione and glutathione peroxidase activity in the heart and liver also returned to baseline levels following the addition of melatonin to Adriamycin. Sodium selenite preserves rBM-MSCs' stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway. Plasma selenium in specific and non-specific forms. USA 106, 1140011405 (2009). Introduction. g, Immunoblots of GPX4, SEPHS2 and Hif1a in MDAMB231 cells subjected to hypoxia (1% O2) for 48hrs. The https:// ensures that you are connecting to the Springett G.M., Husain K., Neuger A., Centeno B., Chen D.-T., Hutchinson T.Z. Selenium can also be used as an adjuvant for chemotherapy and radiation therapy [178,179]. FOIA Traber M.G., Leonard S.W., Bobe G., Fu X., Saltzman E., Grusak M.A. 8600 Rockville Pike These data suggest that melatonin has potential to mitigate negative side effects often experienced by cancer patients throughout the course of treatment and further enhances the notion that melatonin may mitigate normal tissue injuries as well. Sodium selenite inhibits the disulfide exchange on the surface of cancer cell membranes, and thus makes it susceptible to destructive activity of phagocytic cells. Evid Based Complement Alternat Med. All reported effects were independent of cancer type and no severe adverse events associated with melatonin were reported. WebIn conclusion, sodium selenite is a readily available and inexpensive drug of choice in the cancer treatment and prevention. Bookshelf Values are relative to the same cell lines expressing nontargeting guide (black bars), set at 1.0. j, Immunoblots of selenoprotein expression in MCF10A and MDAMB231 cells subjected to (CRISPR/Cas9-induced) KO with guides against SEPHS1, SEPHS2, or control guides for 11 days. WebThe mechanisms of sodium selenite-induced cell death in cervical carcinoma cells were studied during 24 h of exposure in the HeLa Hep-2 cell line. Evaluation of the safety and efficacy of the first-line treatment with somatostatin combined with melatonin, retinoids, vitamin D3, and low doses of cyclophosphamide in 20 cases of breast cancer: a preliminary report. They found that melatonin significantly increased both complete and partial remission (16.5% and 32.6%, respectively), increased the 1-year survival rate from 28.4% to 52.2% with a relative risk of 1.9 (95% confidence interval: 1.282.83), and decreased radiotherapy-related side effects including thrombocytopenia (19.7% vs. 2.2%, relative risk=0.13), neurotoxicity (15.2% vs. 2.5%, relative risk=0.19), and fatigue (49.1% vs. 17.2%, relative risk=0.37). Selenium, administered in the form of sodium selenite, is reduced to hydrogen selenide (H2Se) in the presence of glutathione (GSH) and subsequently generates superoxide radicals upon reaction with oxygen. Structurally, tocopherol and tocotrienols isoforms are similar but tocopherols have a saturated side chain while tocotrienols have 3 double bonds on the side chain (Fig. Ingold, I. et al. Such polythiols associated with cancer membrane-bound proteins appear under the reducing conditions of hypoxic tumor tissue. Increased levels of ROS significantly contribute to: genomic instability, inability to perform differentiated function, immortalization, uncontrolled cellular proliferation, and the progression to the malignant state [2,3,[5], [6], [7], [10], [11]]. The superoxide radical can then be dismuted by superoxide dismutase (SOD) to produce H2O2 and O2 (Equation (3)). Lu J., Kaeck M., Jiang C., Wilson A., Thompson H. Selenite induction of DNA strand breaks and apoptosis in mouse leukemic L1210 cells. Wilson K.M., Mucci L.A. Published by Elsevier GmbH.. All rights reserved. volume2,pages 603611 (2020)Cite this article. Thus, the inorganic Se compound, sodium selenite (SeL), due to its prooxidant character, represents a promising alternative for cancer therapy. Docetaxel and sodium selenite are well known for their anticancer properties. Bethesda, MD 20894, Web Policies This same cohort was also analyzed for the incidence of urinary tract cancer (urothelial cancer and renal cell cancer) and again showed no beneficial effect of vitamin E on cancer initiation [112]. Extended Data Fig. Med. Lung cancer cells transfected with empty plasmids were used as the corresponding controls. Accessibility Sodium selenite is a small water soluble molecule so it has excellent bioavailability. In this review we describe the potential mechanisms and clinical applicability in cancer therapy of four small molecule redox active agents: melatonin, vitamin E, selenium, and vitamin C. Each has shown the potential to have pro-oxidant effects in cancer cells while retaining antioxidant activity in normal cells. Princ F.G., Maxit A.G., Cardalda C., Batlle A., Ana Juknat A. Selenius M, Fernandes AP, Brodin O, Bjrnstedt M, Rundlf AK. 2005 Sep;106(3):193-203. doi: 10.1385/BTER:106:3:193. c, Viability of control and SLC3A2 KO U251 cells treated with 12M of sodium selenite for 48hr. PMC The sulfiredoxin-peroxiredoxin (Srx-Prx) axis in cell signal transduction and cancer development. Med. The altered metabolism of tumor cells was first reported by Otto Warburg in 1927 [12]. Dr. Vivash said sodium selenate activated protein phosphatase 2 A (PP2A), an enzyme that controls the cell cycle as well as cell death (apoptosis), within the brain. 2, 401404 (2012). The peroxiredoxins overlap with the glutaredoxins in the antioxidant recycling system, as described by Ng et al. Selenite at the employed concentrations of 5 and 50 micromol/L produced time- and dose-dependent suppression of DNA synthesis and induced DNA damage which resulted in phosphorylation of histone Purpose: Sodium selenite (SS) has been widely reported to induce apoptosis in various cancer cell types. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). This would alleviate the side-effects of the treatment. physical therapy, pharmacological Jiang, C., Wang, Z., Ganther, H. & Lu, J. Sodium selenite (SS), a common inorganic selenium, can induce superoxide anion production and lead to cancer cell apoptosis [17, 18]. Effects of Sodium Selenite Injection on Serum Metabolic Profiles in Women Diagnosed with Breast Cancer-Related Lymphedema-Secondary Analysis of a Randomized Placebo-Controlled Trial Using Global Metabolomics. 280, 3742337429 (2005). Tan D.-X., Manchester L.C., Reiter R.J., Plummer B.F., Hardies L.J., Weintraub S.T. Generally, organic selenium compounds, e.g. For therapeutic purposes, the dose for sodium selenite ranges from 6,000-30,000 micrograms per day. Sodium Selenite Market Increasing applications of sodium selenite in cancer is expected to boost the growth of the market. ICP-MS-based experiments for selenite measurements were conducted by M.S. published a meta-analysis of all randomized, controlled clinical trials utilizing melatonin in cancer patients with solid tumors [78]. Kim, J. Y. et al. Sodium selenite (SSE) has been reported to exert anti-tumor effects in several cancer cells. Selenium drives a transcriptional adaptive program to block ferroptosis and treat stroke. Melatonin (N-acetyl-5-methoxytryptamine) has been suggested to be a compound capable of preventing and treating several cancer types by suppressing pathways associated with the hallmarks of cancer, including the regulation of pro-survival signaling and tumor metabolism, the inhibition of angiogenesis and metastasis, and the induction of epigenetic alterations in oncogenes and tumor suppressors. showed cytotoxicity of sodium selenite on both cancerous and non-cancerous colon cells . McLaughlin P.J., Weihrauch J.L. The melatonin receptor, MT2, has recently been shown to have differential expression among lung cancer subtypes, with higher MT2 expression being associated with a more favorable prognosis [47]. The chemotherapy agent, adriamycin, is associated with significant heart and liver toxicity via oxidative damage. Physiological concentrations of melatonin have been shown to decrease the growth rate of breast cancer cells by increased expression of p21/WAF1 along with p53 [34]. Selenium (Se) is a ubiquitous, albeit not uniformly distributed metalloid present in earth crust. Pratt D.A., Tallman K.A., Porter N.A. For c,d n=3 biological replicates; error bars are S.D. Hondal R., Motley A., Hill K., Burk R. Failure of selenomethionine residues in albumin and immunoglobulin G to protect against peroxynitrite. WebHowever, the Se-induced epigenome in breast cancer cells was yet to be studied. Serum levels of IGF-1 were decreased by the combination therapy. Careers. 2018 Nov;37(45):5967-5981. doi: 10.1038/s41388-018-0373-2. Electric power use and breast cancer: a hypothesis. Values are relative to that of the same cells treated with vehicle (=1.0). 404, 115120 (2007). Therefore, we currently think the most optimal way to effectively deliver pharmacological doses of ascorbate is intravenously. Madsen M.T., Hansen M.V., Andersen L.T., Hageman I., Rasmussen L.S., Bokmand S. Effect of melatonin on sleep in the perioperative period after breast cancer surgery: a randomized, double-blind, placebo-controlled trial. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Nutr. An oral dose of 300mg -, -, and -tocotrienol has been shown to have a plasma halflife of 4.4, 4.3, and 2.3h, respectively [143]. [Modulation of the inflammatory response through complement-neutrophil activation feedback mechanism with selenium and vitamin E]. Copyright 2016 The Authors. Chem. Morphol. However, selenium treatment may affect microRNA (miRNA) expression as the pattern of Dr. Vivash said sodium selenate activated protein phosphatase 2 A (PP2A), an enzyme that controls the cell cycle as well as cell death (apoptosis), within the brain. Along with iron, there is also evidence of melatonin being able to decrease copper-mediated DNA damage in vivo [30]. These data all point to ascorbate being a pro-oxidant by the formation of high fluxes of H2O2 upon its oxidation. Also in 2014, a phase 1 clinical trial in ovarian cancer showed that pharmacological ascorbate enhanced chemosensitivity in murine models and protected against carboplatin and paclitaxel chemotherapy-associated toxicity in human subjects [215]. a, Viability of non-transformed (blue bars) and selenophilic cancer (red bars) cells under hypoxia (1% O2) for 48hr. conducted mining and analyses of TCGA and other large-scale datasets. Yanagawa T., Ishikawa T., Ishii T., Tabuchi K., Iwasa S., Bannai S. Peroxiredoxin I expression in human thyroid tumors. The pro-oxidant nature of melatonin may be partly due to disruptions in the electron transport chain (ETC), a hypothesis based on the preferential accumulation of melatonin in the mitochondria [19,52]. Despite the great promise for the use of melatonin as an adjuvant to cancer therapy, further investigation into the utility of melatonin, both mechanistically and translationally, is warranted. government site. contracts here. Pharmacokinetics and bioavailability of -, - and -tocotrienols under different food status. SS has been highlighted as a Lee D.J., Kang D.H., Choi M., Choi Y.J., Lee J.Y., Park J.H. PubMed Regulation of ferroptotic cancer cell death by GPX4. The flow of electrons occurs via the reductase/glutaredoxin pathway (GR/GRX), and the Se-dependent thioredoxin/thioredoxin reductase (Trx/TrxR) pathway [[106], [107], [108], [109]]. sharing sensitive information, make sure youre on a federal National Library of Medicine Various organic and inorganic Se compounds, generally considered to be antioxidants, produced mixed results when tested in animal models and human subjects. : Off. Acta 1512, 335344 (2001). In 2005, Mills et al. Park S.K., Sanders B.G., Kline K. Tocotrienols induce apoptosis in breast cancer cell lines via an endoplasmic reticulum stress-dependent increase in extrinsic death receptor signaling. Wallenberg M., Misra S., Wasik A.M., Marzano C., Bjrnstedt M., Gandin V. Selenium induces a multi-targeted cell death process in addition to ROS formation. Cerami, E. et al. There are several natural forms of vitamin E, namely, -,-,-, tocopherol (TOH) and tocotrienol (TE-OH) [89] and have been suggested to have both antioxidant effects in normal tissues as well as pro-oxidant effects in cancer cells [90]. Inflammation; Lymphedema; Lymphedema therapy; Oxidative stress; Selenium; Sodium selenite. Tan D.-X., Hardeland R., Manchester L., Galano A., Reiter R. Cyclic-3-hydroxymelatonin (C3HOM), A potent antioxidant, scavenges free radicals and suppresses oxidative reactions. Would you like email updates of new search results? b, ROS quantification of cells treated with indicated concentrations of sodium selenite or 2xselenocysteine containing peptide, 250M TBH, or 500M H2O2 for 45mins. 2006 Mar 10;160(1):1-40. doi: 10.1016/j.cbi.2005.12.009. Oberley L.W., Buettner G.R. showed that 1mM melatonin induced ROS production as early as 1min following exposure with ROS and persisting up to 6h post-treatment [44]. -Tocopherol or combinations of vitamin E forms induce cell death in human prostate cancer cells by interrupting sphingolipid synthesis. eCollection 2023. Tocotrienol-induced caspase-8 activation is unrelated to death receptor apoptotic signaling in neoplastic mammary epithelial cells. The .gov means its official. Pharmacotherapy, for example with diuretics, has received little attention, because they were not effective and only offered short-term solutions. WebSodium selenite (SSE), a source of inorganic selenium, has been widely used as a clinical cancer treatment, but the precise molecular mechanisms of SSE remain to be elucidated. Staining is as follows: (a) unlabeled, (b) PI, (c) Annexin V, (d) Annexin V and PI, (e) Annexin V and PI stained cells treated with 50M H2O2 for 24hr. Pavlova N.N., Thompson C.B. PMC 2022 Dec 29;15:7799-7810. doi: 10.2147/IDR.S393434. In this way selenite prevents non-enzymatic formation of parafibrin that coats tumors cells and hence presents them as 'self' to the innate cellular immune system. AFMK is the primary product in aerated solutions (84%), while HO-melatonin is prevalent in low O2 environments (86%). Simultaneous detection of the enzyme activities of GPx1 and GPx4 guide optimization of selenium in cell biological experiments. SS dramatically repressed expression of SOX2 and its upstream regulator GLI1 and strongly decreased stemness sphere formation in LAD cells at 10 M. are listed as the authors on a patent application filed by University of Massachusetts Medical School on targeting SEPHS2 in cancer therapy, and on the methodology for hydrogen-selenide detection. The cancer-derived cells obtained from prostate cancer patients were significantly more sensitive to selenite-induced apoptosis than the corresponding normal cells . Breast and other cancer cells are selenophilic, owing to a secondary function of the cystine/glutamate antiporter SLC7A11 that promotes selenium uptake and
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